Aphidicolin: A Specific Inhibitor of DNA Synthesis in Synchronous Mastocytoma P-815 Cells
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منابع مشابه
Arachidonic acid mobilization among phospholipids in murine mastocytoma P-815 cells: role of ether-linked phospholipids.
The ethanolamine-containing glycerophospholipids, choline-containing glycerophospholipids, and phosphatidylinositol fractions are major sources of arachidonic acid in murine mastocytoma P-815 cloned cells. The choline-linked fraction contained high arachidonic acid contents in 1-O-alkyl-2-acyl- (18%) and 1,2-diacyl-sn-glycero-3-phosphocholine (11%), with smaller amounts in 1-O-alk-1'-enyl-2-acy...
متن کاملArachidonic acid mobilization among phospholipids in murine mastocytoma P-815 cells: role of et her4 in ked phospholipids
Abrtract The ethanolamine-containing glycerophospholipids, choline-containing glycerophospholipids, and phosphatidylinmitol fractions are major sources of arachidonic acid in murine mastocytoma P-815 cloned cells. The choline-linked fraction contained high arachidonic acid contents in 1-0-alkyl-2-acyl(18%) and 1,2-diacyl-sn-glycero-3-phosphocholine (ll%), with smaller amounts in l-O-alk-l'-enyl...
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Aphidicolin, a fungal metabolite which is a specific inhibitor of DNA polymerase alpha, inhibited the incorporation of [14C]acetate into desmosterol in mouse L cells by 50% at a concentration of 8.8 microM. It had no effect on acetate metabolism into fatty acids or CO2. The site of inhibition was determined to be distal to the formation of mevalonic acid since aphidicolin also inhibited the inc...
متن کاملThe effect of aphidicolin on adenovirus DNA synthesis.
Aphidicolin inhibits adenovirus DNA replication in HeLa cells and in a cell-free, infected, nuclear extract in which viral DNA is elongated. The compound inhibits alpha DNA polymerase, extensively purified from HeLa cells, but has little or no effect on the beta or gamma DNA polymerases similarly purified. Aphidicolin does not affect thymidine uptake by cells nor does synthesis as it also inhib...
متن کاملDifferential effect of aphidicolin on adenovirus DNA synthesis and cellular DNA synthesis.
There is strong evidence for a participation of DNA polymerase gamma in the replication of adenovirus (Ad) DNA. To study a possible additional role of DNA polymerase alpha we measured the effect of aphidicolin on viral DNA replication. In intact cells, aphidicolin inhibits Ad DNA synthesis weakly. The drug concentration required for 50% inhibition of Ad DNA replication was 300-400 fold higher t...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1980
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)31465-9